Fig 2.

In vitro HDP/CAP susceptibility assays following CCCP exposure. The strains tested were the S. aureus UAMS-1 parent, the ΔlytS mutant, the ΔlytS mutant containing the lytSR complementation plasmid (ΔlytS Comp), the ΔlrgAB mutant, and the ΔlrgAB mutant containing the lrgAB complementation plasmid (ΔlrgAB Comp). Cells were grown to mid-exponential phase (2.75 h) and then treated with CCCP (10 μM) for 15 min. The CCCP-treated cells were then assessed for hNP-1 (A) and DAP (B) susceptibility profiles compared to CCCP-unexposed cells. White bars, CCCP-free cultures; gray bars, CCCP-treated cultures. *, P < 0.01; **, P < 0.05.