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Effects of glucose concentration and pH on PfHK activity. (A) PfHK (200 ng) activity in response to increasing glucose concentration. (B) The pH profile of PfHK (200 ng) activity. Three different buffers with buffering capacity in the range of pH tested were used for the assay: piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES) (pH 6.2, 6.5, and 7.0), triethanolamine (pH 7.4), and Tris (pH 8.0 and 8.8). Reactions were carried out in triplicate, and standard deviations are indicated.
