Fig 6.

BAL fluid analysis. BAL fluids were harvested 24 h after infection with P. aeruginosa PAO1/pMJT1, PAO1/pMJT1-aiiM, or uninfected control. Total cell counts (a), differential cellular analysis (b), and MPO levels (c) in cell-free BAL fluids are shown. Each bar represents average values, and the error bars show the standard errors of the means (n = 8 mice per group). Both P. aeruginosa PAO1/pMJT1 and PAO1/pMJT1-aiiM induced a similar increase in the total BAL fluid cell and neutrophil numbers in infected mice. The levels of MPO in cell-free BAL fluids were significantly reduced in the mice infected with P. aeruginosa PAO1/pMJT1-aiiM. Neu, neutrophils; Mac, macrophages; Lym, lymphocytes.