Table 1.
Bacterial strains and plasmids used
| Strain or plasmid | Genotype | Source |
|---|---|---|
| P. aeruginosa | ||
| PAO1 | Wild-type prototroph | 12 |
| PAO1/pMJT1 | PAO1 with pMJT1 | 15 |
| PAO1/pMJT1-aiiM | PAO1 with pMJT1-aiiM | This study |
| C. violaceum | ||
| CV026 | ATCC 31532 derivative; cviI::Tn5xylE; Kmr, Smr | 11 |
| VIR07 | ATCC 12472 derivative; cviI::Kmr; Ampr | 11 |
| Plasmid | ||
| pUC118-aiiM | pUC118 (cloning vector; TaKaRa Bio) containing homoserine lactonase gene aiiM; Ampr | 11 |
| pMJT1 | Broad-host-range expression vector containing the araBAD promoter and the araC gene; Ampr, Carr | 15 |
| pMJT1-aiiM | pMJT1 with arabinose-inducible aiiM; Ampr, Carr | This study |