Fig 5.
Infectivity and surveillance of Leishmania lines in mouse peritoneal macrophages. Infection of mouse peritoneal macrophages with stationary wild-type (WT), pXG (control), ABCI4, ABCI4K/M, and reverted (ABCI4rev) Leishmania lines using a macrophage/parasite ratio of 1:5 was performed as described in Materials and Methods. The percentage of infected macrophages (A) and the mean number of parasites per macrophage (B) were determined 24 h (black histograms) and 72 h (gray histograms) postinfection. The data are the means ± the SD of three independent experiments. Significant differences versus the control line were determined by using the Student t test (*, P < 0.01 versus respective control).