Fig 4.

Effects of TP10 on membrane integrity. Membrane integrity was analyzed by fluorescence microscopy of N. meningitidis in the presence of the cell-impermeable nucleic acid stain SYTOX green, which exhibits greatly enhanced fluorescence upon uptake and DNA binding. FAM20 cells were grown to log phase in GC broth. After the addition of SYTOX green (5 μM) and TP10 (0, 4, or 8 μM), bright-field and fluorescence images (SYTOX green) were captured at the indicated time points and with fixed exposure settings. Representative images are shown.