Table 1.
Genetic analysis of mutants partially resistant to G2cpsa
| Mutant | Insert | Genome insertionb | Relevant information | Cellular location |
|---|---|---|---|---|
| C | Tn | 4499672, 1,954 bp before yjhB | EcoGene accession no. EG12544 (1,218 bp), uncharacterized member of the major facilitator superfamily of transporters (49) | M |
| Tn | 4499672, 515 bp after yjgZ | EcoGene accession no. G7899 (330 bp), KpLE2 phage-like element; predicted protein | ND | |
| Mu | 1969684, 1,031 bp within tar | EcoGene accession no. EG10988 (1,662 bp), chemoreceptor that senses aspartate and exists as a functional homodimer (50) | M | |
| D | Tn | 2531759, 25 bp before ptsH | EcoGene accession no. EG10788 (258 bp), phosphohistidinoprotein-hexose phosphotransferase, the second of two sugar-nonspecific protein constituents of the phosphotransferase system (51) | C |
| Mu | 1485199, 60 bp before ydcF | EcoGene accession no. EG12110 (801 bp), potential S-adenosylmethionine-dependent enzyme which may play a role in anaerobic respiration (52) | ND | |
| E | Tn | 839158, 1,596 bp within ybiL | EcoGene accession no. G6414 (2,283 bp), putative outer membrane receptor for iron transport (53) | OM |
| Mu | 1984638, 487 bp before araF | EcoGene accession no. EG10057 (990 bp), member of the arabinose ABC transporter (54) | P | |
| Mu | 1984638, 310 bp before yecI | EcoGene accession no. G7033 (504 bp), predicted ferritin-like protein | C | |
| L | Tn | 951258, 1,519 bp within pflB | EcoGene accession no. EG10701 (2,283 bp), pyruvate formate-lyase (inactive) (55) | C/M |
| Mu | 3089457, 304 bp within yggJ | EcoGene accession no. EG12366 (732 bp), methyltransferase responsible for methylation of 16S rRNA at the N-3 position of the U1498 nucleotide (56) | C | |
| M | Tn | 4321916, 14 bp within phnD | EcoGene accession no. EG10714 (1,017 bp), periplasmic binding component of the alkylphosphonate ABC transporter (cryptic in E. coli K-12) (57) | P/OM |
| Mu | 2076442, 313 bp after yeeW | EcoGene accession no. G7086 (168 bp), CP4-44 prophage, predicted protein | ND | |
| Mu | 2076442, 157 bp after yoeF | EcoGene accession no. G0-10456 (357 bp), conserved protein | ND | |
| P | Tn | 4321916, 14 bp within phnD | See mutant M | P/OM |
| Mu | 2159484, 3,076 bp within yegO | EcoGene accession no. G7115 (3,078 bp), member of the MdtABC-TolC multidrug efflux transport system (58) | OM | |
| Z | Tn | 832367, 74 bp within dinG | EcoGene accession no. EG11357 (2,151 bp), ATP-dependent DNA helicase (59) | C |
| Mu | 1068913, 149 bp within ycdG | EcoGene accession no. G6517 (1,329 bp), uncharacterized member of the NCS2 family of nucleobase transporters (60) | M |
a
Tn, transposon; C, cytoplasmic; M, membrane associated; OM, outer membrane; P, periplasm; ND, not determined. Accession numbers, lengths of potential mutated genes, the function and cellular location of the encoded proteins, and references were obtained from the EcoCyc server (http://www.ecocyc.org/).
b
Numbers correspond to nucleotide positions obtained from the EcoCyc server.