Alignment and phylogenetic analysis of SFV env sequences. (A) Schematic representation of aligned nucleotide sequences of SFVmcy-1 and SFVmcy-2. Gray vertical lines indicate silent mutations, and black vertical lines indicate nonsilent mutations. LP, SU, and TM junctions are shown by arrows. Length is shown in kb. (B to E) Results of phylogenetic analysis of full-length env, using a total of 2,855 positions in the final data set (B), LP, using a total of 369 positions in the final data set (C), SU, using a total a total of 1,303 positions in the final data set (D), and TM, using a total of 1,244 positions in the final data set (E), are shown. BFV was used as an outgroup for all of the trees. The accession numbers of the sequences used for the comparisons are listed in Table 2.