Fig 4.

Alignment of SFV SU regions. Amino acid sequences of SFVmcy-2, SFVmcy-1, SFVagm-3, SFV-R289HybAGM, and SFVmmu-K3T were aligned in MegAlign using ClustalW with the BLOSUM protein weight matrix. Dots indicate identity, and dashes indicate gaps. The glycosylation site found to be required for folding in the prototype foamy virus (PFV; originally designated human foamy virus or HFV) and preserved in all SFVs is labeled with asterisks. Residues predicted to be included in the three essential domains of the bipartite receptor binding domain are underlined. The amino acids that correspond to the nucleotides predicted for the recombination breakpoint sequences by SimPlot 3.5.1 are boxed as follows: solid line, SFVmcy-2; broken line, SFV-R289HybAGM.