Fig 5.

Susceptibility of TW10-translocated HIV-1 to TW10-specific CTLs. As described in Materials and Methods, the suppression efficiency of the TW10-specific CTLs for the HIV-1 variants indicated was evaluated by inhibition assays. (A) The mean amount of virus suppression efficiency (based on the log₁₀ p24 concentration [pg/ml] on day 7) for each virus indicated is plotted for three TW10-specific CTL clones. The error bars depict the standard deviations of the triplicates within each experiment and across all of the experiments with the same CTL clone. (B) The mean level of suppression of each virus indicated across all of the clones tested is plotted, and the error bars depict the standard deviations across all of the clones tested. (C) The Nef impact factor is plotted for the TW10 epitope expressed endogenously in Gag (Late) versus that translocated in Nef (Early). Each dot represents an independent experiment with a TW10-specific CTL clone. The horizontal line indicates the mean of individual experiments in each group, and the P value is the result of a two-tailed Student t test.