Fig 6.
Processing efficiency of Gag epitopes translocated to Nef. Synthetic peptides corresponding to the Gag epitopes and their flanking sequences after translocation to Nef were incubated with PBMC cytosolic extracts, and the resulting degradation peptides were analyzed by mass spectrometry. (A) The percentages of peptide cleavage products representing the minimal epitopes, partially processed epitopes (cleaved at the N or C terminus), unprocessed epitopes (not cleaved at either terminus), and disrupted epitopes are indicated on a log10 scale. (B) Full list of the cleavage products detected. The top row indicates the input peptide.