Fig 1.

Construction of Ac-MK, Ac78KO, Ac78Re, and GP64KO bacmids. (A) Schematic diagram of Ac-MK, Ac78KO, Ac78Re, and GP64KO bacmids. The ac78 gene and gp64 gene were knocked out by insertion of the pUC ori and Amp into amino acid 87 of AC78 and amino acid 337 of GP64, respectively, via Tn7-mediated transposition. The deletion of ac78 was repaired by replacement of Kan with ac78 and Cm via homologous recombination between pUC-19-MCP-ac78 and Ac78KO. The ac78 gene inserted into Ac78Re is driven by its own promoter. (B) RT-PCR analysis of ac78 and gp64 transcription. Total RNA was extracted from transfected Sf9 cells at 72 h p.t. Lanes: M, 100-bp ladder; 1, mock-transfected Sf9 cells; 2, Ac-MK-transfected Sf9 cells; 3, Ac78KO-transfected Sf9 cells; 4, GP64KO-transfected Sf9 cells.