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Transcription of ac78 in Sf9 cells. Total RNA was extracted from Sf9 cells infected with Ac-MK at 0, 0.5, 1, 2, 6, 12, 24, 48, and 72 h p.i. and subjected to qPCR analysis using an ac78-specific primer set. 28S rRNA was used as a reference gene. The error bars indicate standard deviations.
