Fig 6.

Western blot analysis of AC78 in purified and fractionated virions. BV and ODV were purified using a sucrose gradient and analyzed by SDS-PAGE and Western blotting. The blots were probed individually with an anti-Ac78 antibody to detect AC78, anti-AcV5 monoclonal antibody to detect the BV envelope protein GP64, and anti-VP39 to detect the nucleocapsid protein VP39. NC, nucleocapsid fraction; E, envelope fraction. GP64 and VP39 were analyzed to confirm correct fractionation of the BV particle in envelope and nucleocapsid fractions, respectively.