Fig 4.

Analysis of TF mutants in a mouse model of SINV neuropathogenesis. Two-week-old CD-1 mice were inoculated intracerebrally with 1,000 PFU of the virus indicated. (A) The survival rates are shown as a function of time. (B) Morbidity was scored on a 4-point scale: a score of 0 was given for no obvious symptoms, a score of 1 was given for kyphoscoliosis and ruffling, a score of 2 was given for one hind limb paralyzed, a score of 3 was given for two hind limbs paralyzed, and a score of 4 was given for death. (C) Two-week-old CD1 mice were infected intracranially with 1,000 PFU of wild-type or ΔTF virus. Brains were harvested at 3 and 5 dpi, and 10% (wt/vol) homogenates were made in PBS using one hemisphere of the brain and further clarified. The homogenate was titrated on BHK-21 cells to assess viral titers.