Fig 2.

PDTC's antiviral property was mediated by its dithiocarbamate moiety, and this antiviral effect was reversible after removal of PDTC. (A) The dithiocarbamate moiety of the PDTC molecule was essential for its antiviral property. HEC-1-A cells were exposed to 300 μM PDTC, DDTC, or pyrrolidine prior to HSV-2 infection (MOI = 1). (B) PDTC's antiviral effect was reversible. HEC-1-A cells were exposed to PDTC (25 μg/ml) prior to infection. After 3 h of incubation, PDTC was removed by washing and complete medium was supplied. The gD expression levels were determined 24 h p.i. via the In-Cell Western assay and normalized to the β-catenin level. Error bars show standard deviations.