Fig 4.

Effect of PDTC on the expression of HSV IE proteins. (A, B) PDTC inhibited the expression of HSV-1 ICP0 (A) and ICP4 (B). HEC-1-A cells exposed to PDTC were infected with HSV-1 (MOI = 1). ICP0 and ICP4 expression levels were determined 24 h p.i. and normalized to the β-catenin level. (C) PDTC effects on HSV-1 ICP0 and ICP4 expression at different time point were determined via Western blot assay. (D) PDTC effects on HSV-1 ICP0 and ICP4 mRNA transcription at 6 h or 12 h p.i. ICP0 and ICP4 mRNA expression levels were quantified via real-time PCR. (E) PDTC interfered with nuclear-cytoplasmic translocation of ICP0. HEC-1-A cells were infected with HSV-1 (MOI = 1) for 2 h and then incubated with PDTC (25 μg/ml) or MG132 (6 μg/ml). ICP0 localization was observed via immunofluorescence staining 12 h p.i. All experiments were performed 2 to 3 times, and a representative result is shown. Error bars show standard deviations.