Table 1.
Primers used in this study
| Coronavirus | Primer |
|
|---|---|---|
| Forward | Backward | |
| Ty-BatCoV HKU4 | ||
| RdRp | LPW3283 5′-GTAATGTCTGTCAGTATTGGGTT-3′ | LPW3232 5′-AACTAATATGCTCTTTAACACTTCAC-3′ |
| LPW2771 5′-TGYTAYGCTTTAMGNCAYTTYGA-3′ | LPW2773 5′-GTTGGGTAATAACAAAATCACCAA-3′ | |
| LPW2626 5′-GTTTTAACACTYGATAAYGARGA-3′ | LPW2630 5′-AGTATATTGAARTTNGCACARTG-3′ | |
| LPW2738 5′-CCACCCTAATTGTGTTAATTGTA-3′ | LPW2775 5′-TAACTGAAGACCCTTCCTTGAAA-3′ | |
| LPW3233 5′-GGCAATTTTAATAAAGATTTTTATGA-3′ | LPW3234 5′-GCCAAAATCAATGACGCTAAAAT-3′ | |
| LPW1507 5′-GGTTGGGACTATCCTAAGTGTGA-3′ | LPW1508 5′-CCATCATCAGATAGAATCATCATA-3′ | |
| LPW1037 5′-WTATKTKAARCCWGGTGG-3′ | LPW1040 5′-KYDBWRTTRTARCAMACAAC-3′ | |
| LPW3235 5′-CTTAATAAACACTTTTCTATGATGAT-3′ | LPW2678 5′-TACTCACCGAGCTGTACTTTACTA-3′ | |
| S | LPW3797 5′-AGATTTATATAAAATTATGGGAA-3′ | LPW4102 5′-TACGTGGTTTTAATATGCAATAAAA-3′ |
| LPW3899 5′-TCTCTTACTAATACATCGGCT-3′ | LPW3900 5′-AAGACCTGACCATCTTCAGAAA-3′ | |
| LPW4103 5′-TGGTGCAAACCAAGATGTTGAAA-3′ | LPW3712 5′-CTAGCGCTATAACTTCTAAAAGTA-3′ | |
| LPW3720 5′-CATTAGTAGTTAGTGATTGTAAA-3′ | LPW2821 5′-GTCATAAAGTGGTGGTAAAACTT-3′ | |
| LPW2319 5′-ATTAATGCTAGAGAYCTHMTTTG-3′ | LPW2320 5′-TTTGGGTAACTCCAATNCCRTT-3′ | |
| LPW2824 5′-TTTGCCGCTATACCTTTTGCACAA-3′ | LPW4106 5′-TGAGTTATAGGTTCAGGTTTATAA-3′ | |
| LPW4105 5′-TATTAGTGACATCCTTGCTAGGCTT-3′ | LPW2317 5′-GAGCCAAACATACCANGGCCAYTT-3′ | |
| LPW4107 5′-ATGGTCCTAACTTTGCAGAGATA-3′ | LPW21565 5′-TGCCAGACATGCCACCACAA-3′ | |
| N | LPW21407 5′-AACGAATCTTAATAACTCATTGTT-3′ | LPW21408 5′-CTCTTGTTACTCTTCATTGGCAT-3′ |
| Pi-BatCoV HKU5 | ||
| RdRp | LPW3350 5′-TTTGTCAATTTTGGATAGGACAT-3′ | LPW3352 5′-TGATGCATCACAGCARCCATA-3′ |
| LPW3351 5′-ATCAGAATAACTGTGAAGTGCTT-3′ | LPW3275 5′-GACAATTGGACCAAAAGACGTT-3′ | |
| LPW3382 5′-CAAATTGTGTGAACTGTACTGAT-3′ | LPW3387 5′-ATATATCTCGAAGTAACGATCAA-3′ | |
| LPW3172 5′-GTCCTGGCAACTTTAATAAAGATT-3′ | LPW3130 5′-CTAATATGAGAGATGCAAAGA-3′ | |
| LPW1507 5′-GGTTGGGACTATCCTAAGTGTGA-3′ | LPW1508 5′-CCATCATCAGATAGAATCATCATA-3′ | |
| LPW3384 5′-CTAAATTTGTGGACAGGTATTAT-3′ | LPW3399 5′-CTTCGTATACACGTACCACAA-3′ | |
| S | LPW21416 5′-CTCTTGTCGCAGGGTAAACTT-3′ | LPW4284 5′-AAAGACTCTACCTGTGCAGAATA-3′ |
| LPW4086 5′-TAACTTATACTGGACTGTACCCAAA-3′ | LPW4193 5′-AAGCCATTTGAAGGTTACCATT-3′ | |
| LPW4192 5′-ACTTTGCTACTTTACCTGTGTAT-3′ | LPW4137 5′-AGTAACACCAAATGTGAAATT-3′ | |
| LPW4285 5′-AATCGCCACTCTAAACTTTACTA-3′ | LPW4286 5′-AAGAGGCTGGGTATTCTGGGTT-3′ | |
| LPW4138 5′-AAGATGAGTCTATTGCTAATCTAT-3′ | LPW4139 5′-AGCTTCCATATAGGGGTCATA-3′ | |
| LPW4287 5′-TGTGCACAATATGTTGCTGGCTA-3′ | LPW4288 5′-AAAGAACTACCAGTATAATACCAA-3′ | |
| LPW4140 5′-AACACTGAGAATCCACCAAA-3′ | LPW21417 5′-CACACGCATCATAAGTTCGTT-3′ | |
| N | LPW21361 5′-GAATCTTATTATCTCATTGTT-3′ | LPW21362 5′-CTATTACGTTCAATTGGCAAT-3′ |