Fig 6.

mRNA expression analysis of mitochondrial biomarkers (A and B) and protein expression analysis of a complex II subunit (C and D) in RV-infected Vero and A549 cells. RT-qPCR was applied for the analysis of the gene expression of the nuclear transcription factors NRF1 and -2 (A) and of nucleus-encoded subunit NDUFB1 of complex I, SDHA and SDHB of complex II (succinate dehydrogenase), subunit UQCRC1 of complex III, and COX4I1 of complex IV (B). Data are shown as percentages of the corresponding mock-infected controls. Gene expression was normalized against HUEL and HPRT-1. The mitochondrial protein expression level of SDHA was resolved by a 10% SDS-PAGE using 70 μg of mitochondria isolated from RV- and mock-infected Vero (C) and A549 (D) cells. The blot was probed with SDHA and VDAC (loading control) antibodies. Level of significance from the control population is indicated by asterisks (*, P < 0.05; **, P < 0.01; and ***, P < 0.001).