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. 2013 Aug;87(15):8624–8637. doi: 10.1128/JVI.00899-13

Fig 4.

Fig 4

Genotyping of eBSOLV-1 and eBSOLV-2 in the F1 triploid (AAB) population using a dCAP marker. The PCR product obtained from eBSOLV-1 is cut into two bands of 83 and 254 bp by the endonuclease HaeIII (New England BioLabs), whereas the one from eBSOLV-2 is not digested. Lane M, 1-kb DNA ladder (Invitrogen); lane 1, undigested PCR product of the diploid M. balbisiana PKW; lanes 2 to 6, digested PCR product from PKW carrying both eBSOLV alleles, BAC MBP_31007 carrying eBSOLV-1, BAC MBP_73B22 carrying eBSOLV-2, and two triploid (AAB) F1 progeny of a genetic cross between PKW and M. acuminata cv. IDN 110 4x (AAAA) carrying eBSOLV-2 and eBSOLV-1, respectively. Digested DNA was loaded onto a 2.5% agarose gel stained with ethidium bromide, and bands were visualized under UV light.