Table 1.
Primers and probes used in qRT-PCR for EW or RRV gene identification or intestinal virus quantitation
| Virus genea | Sequence |
|---|---|
| EW VP7 (gene 9) F | 5′-TCAACCGGAGACATTTCTGA-3′ |
| EW VP7 (gene 9) R | 5′-TTGCGATAACGTGTCTTTCC-3′ |
| RRV VP7 (gene 9) F | 5′-ACGGCAACATTTGAAGAAGTC-3′ |
| RRV VP7 (gene 9) R | 5′-TGCAAGTAGCAGTTGTAACATC-3′ |
| EW NSP2 (gene 8) F | 5′-GAGAATGTTCAAGACGTACTCCA-3′ |
| EW NSP2 (gene 8) R | 5′-CTGTCATGGTGGTTTCAATTTC-3′ |
| RRV NSP2 (gene 8) F | 5′-GAGAATCATCAGGACGTGCTT-3′ |
| RRV NSP2 (gene 8) R | 5′-CGGTGGCAGTTGTTTCAAT-3′ |
| EW NSP3 (gene 7) F | 5′-AGGTTTGAGACATCGAAGCAA-3′ |
| EW NSP3 (gene 7) R | 5′-GAGTGATGACCGATTGCAGA-3′ |
| RRV NSP3 (gene 7) F | 5′-TTGAAGAGAAAATGGAAGTAGATACAA-3′ |
| RRV NSP3 (gene 7) R | 5′-TACTTCTCATTAACCCGATGTTTCA-3′ |
| NSP5 (gene 11) F | 5′-CTGCTTCAAACGATCCACTCAC-3′ |
| NSP5 (gene 11) R | 5′-TGAATCCATAGACACGCC-3′ |
| NSP5 (gene 11) probe | 5′-Cy5/TCAAATGCAGTTAAGACAAATGCAGACGCT/IAbRQSp/-3′ |
a
F indicates forward primer, and R indicates reverse primer.