Fig 3.
MEFs produced from HIV-LucTG mice can be used for in vitro analysis of HIV-1 pseudovirus infection. (A) hCCR5 and hCD4 expression on MEFs from an HIV-LucTG mouse (solid line) and a ROSA-Stop-Luc mouse (tinted region) determined by flow cytometry. (B) Luminescence after infection of HIV-LucTG and ROSA-Stop-Luc MEFs with serial dilutions of preparations of YU2-Cre or VSVg-Cre. Infection of HIV-LucTG MEFs was performed in quadruplicate; infection of ROSA-Stop-Luc MEFs was performed in duplicate. Results of a representative experiment for different viral preparations are presented. (C) Luminescence after infection of HIV-LucTG MEFs with YU2-Cre (13 TCID50s; red) or VSVg-Cre (35 TCID50s; blue) in the presence of different antiretrovirals. Dashed lines, luminescence in the absence of antiretrovirals (red, YU2-Cre; blue, VSVg-Cre) and for uninfected MEFs (black, YU2-Cre experiments; gray, VSVg-Cre experiments). Infections were performed in duplicate. IC50s were calculated using a nonlinear regression curve. n.a., not applicable.