Fig 4.
HIV-LucTG mice become infected with HIV-1 pseudovirus after i.p. injection. (A) Representative luminescence 8 days after i.p. injection. (B) Time course of changes in photon flux per second for an omental ROI for HIV-LucTG mice and transgene-negative littermates injected i.p. with YU2-Cre (585 TCID50s) compared to the luminescence of uninjected mice (left) and photon flux per second 8 days after injection (right). Pooled data from two experiments depicted by individual symbols are presented (total n starting on day 3, 7 to 8 [one mouse died during the course of the experiment] for HIV-LucTG mice, 8 for negative mice, and 2 for uninjected mice). (C) Detection of tdTomato-positive (tdTomato+) cells in peritoneal lavage fluid obtained 6 days after i.p. injection of YU2-Cre (585 TCID50s) in hCCR5+ hCD4+ ROSA26tdTomato/Luc mice or transgene-negative littermates. Representative tdTomato expression found in different cellular subsets of an hCCR5+/hCD4+ mouse is shown. (Left) Gating strategy for cells pregated on the basis of forward scatter and sideward scatter (SSC); (right) mean frequency (n = 3 per group) of tdTomato-positive cells in cellular subsets of hCCR5+/hCD4+ mice and negative littermates. (D) Ex vivo luminescence analysis of dissected omenta after i.p. d-luciferin injection. Analysis was performed 8 days after i.p. YU2-Cre injection (585 TCID50s) in HIV-LucTG mice (left) and 6 days after i.p. VSVg-Cre injection (2,190 TCID50s) in ROSA-Stop-Luc mice (right). Mice were pretreated with antiretroviral compounds starting 24 h prior to viral challenge (efavirenz, 0.5 mg twice p.o.; maraviroc, 1.5 mg twice p.o.; enfuvirtide, 40 μg four times s.c.) or untreated. The log differences in the arithmetic means of photon flux per second defining 0% and 100% infection are 1.2 (YU2) and 3.0 (VSVg). (E) Ex vivo luminescence analysis of dissected omenta after i.p. d-luciferin injection. Analysis was performed 8 days after i.p. YU2-Cre (585 TCID50s) injection in HIV-LucTG mice. Mice were s.c. pretreated with 3BNC117 or 200 μg mGO53 24 h prior to viral challenge. The log difference in the arithmetic mean of photon flux per second defining 0% and 100% infection is 0.74. For panels D and E, untreated or isotype-treated mice were used to define 100% infection, respectively, and naive mice were used to define 0% infection (n ≥ 4 for virus-injected groups, n = 2 to 3 for naive mice).