Table 1.
TIM-1 IgV domain mutants
| Mutation(s) | Entrya | Location | Expression (%)b |
|---|---|---|---|
| K23A | WT | A β-strand | >90 |
| E27A | WT | A β-strand | >90 |
| G29A | WT | AB loop | >90 |
| T33A | WT | B β-strand | >90 |
| P35A | WT | BC loop | >90 |
| H37A | WT | BC loop | >90 |
| Y38A | ▼ | BC loop | >90 |
| S39A | WT | BC loop | >90 |
| S44A | WT | BC loop | >90 |
| S51A | WT | CC′ loop | >90 |
| S53A | WT | CC′ loop | >80 |
| L54A | WT | CC′ loop | >70 |
| F55A | ▼ | CC′ loop | >90 |
| T56A | WT | CC′ loop | >80 |
| Q58A | WT | CC′ loop | >90 |
| N59A | WT | CC′ loop | >90 |
| T64A | WT | CC″ loop | >90 |
| N65A | WT | C′C″ loop | >90 |
| H68A | WT | C′C″ loop | >90 |
| Y71A | WT | C″ β-strand | >90 |
| R72A | WT | C″E loop | >90 |
| K73A | WT | C″E loop | >70 |
| D74A | WT | C″E loop | >90 |
| K78A | WT | C″E loop | >90 |
| L79A | WT | C″E loop | >90 |
| L80A | WT | C″E loop | >90 |
| D82A | WT | C″E loop | >90 |
| R85A | WT | C″E loop | >70 |
| R86A | WT | C″E loop | >90 |
| RR86AA/LL | WT | C″E loop | >90, >90 |
| D87A | WT | C″E loop | >90 |
| S89A | WT | C″E loop | >90 |
| T91A | WT | E β-strand | >90 |
| D99A | ▼ | EF loop | >90 |
| R106A | ▼ | F β-strand | >90 |
| E108A | WT | FG loop | >90 |
| H109A | WT | FG loop | >70 |
| R110A | WT | FG loop | >90 |
| G111A | ▼ | FG loop | >90 |
| W112A | ▼ | FG loop | >90 |
| F113A | ▼ | FG loop | >90 |
| N114A/D/Q | ▼ | FG loop | >90, >90, >90 |
| D115A/E/N | ▼ | FG loop | >50, >90, >90 |
| ND115AA/DN | ▼ | FG loop | >60, >90 |
| M116A | WT | FG loop | >90 |
| K117A | ▼ | G β-strand | >90 |
| E123A | WT | G β-strand | >90 |
a
Entry is assessed by transduction into cells expressing mutant TIM-1 relative to transduction into cells expressing WT TIM-1. “▼” indicates a decrease in transduction.
b
Expression was determined by surface staining with mucin-like-domain-specific MAb AKG7 and grouped into 10% increments.