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. Author manuscript; available in PMC: 2013 Jul 23.
Published in final edited form as: Nature. 2012 Mar 14;483(7389):289–294. doi: 10.1038/nature10838

Figure 3. An IsO-like signalling centre in S. kowalevskii.

Figure 3

a-s, in situ hybridizations for S. kowalevskii and mouse homologs of MHB markers. Arrowheads mark the S. kowalevskii collar/trunk coelom boundary the mouse IsO. a, S. kowalevskii fgf8/17/18 expression. b, mouse fgf8 expression. c, S. kowalevskii wnt1 expression. d, mouse wnt1 expression. e, double FISH showing S. kowalevskii wnt1 (green) expressed directly anterior to fgf8/17/18 (magenta). f, S. kowalevskii otx expression. g, mouse otx2 expression. h, S. kowalevskii gbx expression. Asterisks denote endodermal domains. i, mouse gbx2 expression. j, double FISH for S. kowalevskii otx (green) and gbx (magenta). k, double FISH for S. kowalevskii wnt1 (green) and en (magenta). l, double FISH for S. kowalevskii en and otx. m, mouse en2 expression. n, S. kowalevskii pax2/5/8 expression. o, double FISH showing S. kowalevskii pax2/5/8 and en expression. p-r, expression of mouse pax2 (p), pax5 (q), and pax8 (r). s, double FISH for S. kowalevskii th (green) and en (magenta). t, summary of AP expression topologies in hemichordates and mice. Anterior to top. u, en expression is reduced in an embryo treated with SU5402. v, en expression in a DMSO-treated control embryo. w, en expression in an embryo injected with fgf8/17/18 siRNA. x, en expression in an embryo injected with a control siRNA. y, pax2/5/8 expression in an embryo treated with SU5402. z, pax2/5/8 expression in a DMSO-treated control embryo. ai, pax2/5/8 expression in an embryo injected with fgf8/17/18 siRNA. bi, pax2/5/8 expression in an embryo injected with a control siRNA. ci, en is not expressed in descendants of a blastomere injected with β -catenin siRNA. di, merged darkfield/fluorescence images showing location of the β-catenin deficient clone (green). ei, wild-type en expression. Scale bars = 100 μm in S. kowalevskii embryos and 1 mm in mice. Abbreviations as described previously.