Fig. 2.

MMP activity in FCM is required for paracrine-induced tamoxifen resistance. a LM05-E- and LM05-F-conditioned media were analyzed by gelatin zymography; activities of MMP-9 and MMP-2 were only detected in conditioned media derived from LM05-F cells. b LM05-E cells were cultured in NCM or FCM and treated for 48 h with 10 nM estradiol (E₂) or 10 nM E₂ + 1 µM 4-OH-tamoxifen (E + T). Addition of the broad spectrum MMP inhibitor GM6001 (10 µM) reversed the FCM protective effect, but did not have any effects on its own (***P< 0.001). One of two experiments is shown