Figure 1.

CXCR3 binding chemokines are expressed highly in liver biopsies obtained from primary biliary cirrhosis (PBC) patients. Total RNA was extracted from liver biopsies of PBC patients (n = 20) and normal controls (NCs, n = 20), and multiplex reverse transcription–polymerase chain reaction (RT–PCR) for chemokines and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was performed. (a) Representative PCR blots of mRNA expression from tissue obtained from patients with PBC and NCs, probed for CXCL9/monokine induced by interferon (IFN)-gamma (MIG), CXCL10/IFN-gamma-induced protein-10 (IP-10) and GAPDH. In order to control for genomic contamination each sample has a reverse transcriptase negative control (RT–). The gel shows fluorescence of ethidium bromide-stained PCR products resolved by electrophoresis.