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. 2013 Mar 11;172(1):44–53. doi: 10.1111/cei.12033

Figure 2.

Figure 2

(a) Anti-recombinant human glucose-6-phosphate isomerase (rhGPI) antibody levels. Enzyme-linked immunosorbent assay (ELISA) was performed for rhGPI in rheumatoid arthritis (RA) (n = 158) and healthy control subjects (HS) (n = 71) sera. (b) Anti-rabGPI antibody levels. ELISA assays were also performed for native rabGPI. Data represent the optical density values at 405 nm (OD 405). Each datum point represents an individual subject. Bar indicates the mean value. *P < 0·01; **P < 0·001. (c) Positivity for anti-citrullinated peptide antibodies (ACPA) in anti-whole GPI antibody-positive and -negative patients. Positivity for anti-citrullinated glucose-6-phosphate isomerase peptide-1–9 (CCG-1–9), anti-citrullinated α-enolase peptide (CEP)-1 and anti-cyclic citrullinated peptides (CCP) antibodies was evaluated in anti-rhGPI/anti-rabGPI antibody-positive (GPI +/+; n = 22) and -negative (GPI –/–; n = 88) patients. Positivity of anti-CCG-1, -4, -8 and -9 antibodies in the GPI-positive group was significantly higher than in the GPI-negative group. Data represent the positive percentage of each antibody. *P < 0·05; **P < 0·01. (d,e) Relationship among anti-CCG-2, -4 and -7 antibodies and anti-CEP-1 and anti-CCP antibodies. Anti-CCG-2, -4 and -7 antibodies correlated significantly with anti-CEP-1 antibodies (r = 0·313, P < 0·001; r = 0·264, P < 0·001; and r = 0·400, P < 0·001, respectively) (d). Anti-CCG-2, -4 and -7 antibodies also correlated significantly with anti-CCP antibodies (r = 0·442, P < 0·001; r = 0·333, P < 0·001; and r = 0·458, P < 0·001, respectively) (e). Data represent the OD 405 (anti-CCG-2, -4 and -7 and anti-CEP-1) or RU/ml (anti-CCP). Each datum point represents an individual subject. Solid line indicates the correlation among antibodies.