Figure 3.

All-trans retinoic acid (AT-RA) administration did not have non-specific toxic effects on mononuclear cells. Immunized and unimmunized mice were given AT-RA or only soybean oil treatment (control group) for 4 days. Mononuclear cells were isolated from draining lymph nodes (dLNs) and the toxic effect of AT-RA was measured by analysing the rate of apoptosis. Apoptotic cells were identified as propidium iodide⁺ Annexin-V⁺. Data are representative as mean ± SE of two experiments with five mice in each experiment.