Figure 9.

All-trans retinoic acid (AT-RA) pre-treated bone mesenchymal dendritic cells (BMDCs) abrogated the proliferation of lymphocytes and the differentiation of T helper type 1 (Th1)/Th17 in vitro. AT-RA/DMSO pre-treated BMDCs were incubated with lymphocytes isolated form draining lymph nodes (dLNs) of experimental autoimmune encephalomyelitis (EAE) mice 5 days post-immunization (dpi). Proliferation of lymphocytes (a) was measured by CCK-8 and cytokine production in the population of CD4-positive cells (b) was analysed with flow cytometry. Data, shown as mean ± SE, are representative of two experiments with five mice per experiment. *P < 0·05, **P < 0·01, ***P < 0·001.