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. Author manuscript; available in PMC: 2013 Jul 23.
Published in final edited form as: Annu Rev Biophys. 2012 Feb 23;41:295–319. doi: 10.1146/annurev-biophys-042910-155351

Figure 3. smFRET fluctuations arising from diffusional migration of E. coli SSB on ssDNA.

Figure 3

(a) –(Top) Rapid fluctuations between several FRET states are observed due to diffusion of an SSB tetramer on an 81 nucleotide ssDNA; (bottom) when the available ssDNA is limited to 69 nucleotides by forming a duplex with the 12 nucleotide extension, the FRET fluctuations are severely reduced reflecting less protein diffusion; (b) – anti-correlated FRET time traces from a three color experiment showing SSB tetramer diffusion (Donor (Alexa555)-labeled SSB bound to a (dT)130 DNA labeled at each end with acceptors (Cy5 and Cy5.5) Figures adapted from Ref. (114).