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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Immunol. 2013 Apr 29;190(11):5722–5730. doi: 10.4049/jimmunol.1203343

Figure 1.

Figure 1

MyD88-dependent IL-1β/IL-1R signaling is critical for CFA-induced Th17 polarization. (a) Draining LN cells were harvested from WT or Myd88 −/− mice 14 days after CFA/OVA immunization and intracellular cytokine staining (ICS) for IL-17 and IFN-γ was performed after ex vivo OVA restimulation. Plots are gated on CD4+ CD44hi Foxp3 cells. (b–d) The indicated mice were immunized and draining LN responses were measured as in a. Frequencies of cytokine-producing cells within the CD4+ CD44hi Foxp3 population are shown. Data are representative of at least three independent experiments (a, b) or are pooled from three to four experiments (c, d). Each point represents data from an individual mouse.