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. 2012 Mar 19;14(4):626–634. doi: 10.1038/aja.2011.176

Figure 1.

Figure 1

Cloning of the full-length mHong1. (a) The rat HongrES1 cDNA sequence was used to interrogate the mouse genome. Two fragments (224 bp and 653 bp) with significant similarity were obtained. On the basis of the sequences of these two fragments, two pairs of primers (FP1/RP1, FP2/RP2) were synthesized. (b) Amplification of cDNA fragments of mHong1 by RT-PCR. The cDNA fragments of 224 bp, 653 bp and 1524 bp were amplified with primers FP1/RP1, FP2/RP2 and FP1/RP2, respectively. (c) Northern blot analysis of mHong1 mRNA in the epididymis and testis probed with different cDNA regions (1–224 bp, 225–871 bp and 872–1524 bp). 18s rRNA was used as loading control. Ep, epididymis; Te, testis.