Figure 2.

The cDNA and deduced amino acid sequence analysis and the intron–exon structure of mHong1. (a) The mHong1 open reading frame contains 1257 bp coding for a 418-amino acid protein, and the initial and terminal codons are boxed. The protein contains a putative signal peptide (in italics) with a cleavage site between amino acids 20 and 21. The N-glycosylation (N_{31, 151, 223, 228, 263, 322 and 383}) sites are shadowed grey. The serpin motif from 380th to 390th amino acid is in bold and boxed. (b) The mHong1 gene diagram shows the relative lengths of exons 1–5 and intervening introns. The shadowed grey box represents the open reading frame of mHong1 mRNA. (c) Amino-acid sequence alignment of mouse mHong1 and rat HongrES1. Dashes show alignment gaps. The conserved serpin motif residues are indicated by grey box.