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. 2012 May 14;14(4):536–545. doi: 10.1038/aja.2011.179

Figure 3.

Figure 3

Expression of tumor-associated-genes and proteins in DU145 cells transfected with different treatment plasmids after 48 h. (a) Tumor-associated gene expression in DU145 cells transfected with psi-Survivin, pGRIM-19, pGRIM-19-si-Survivin and psi-Scramble empty vector revealed by RT-PCR. (b) The quantified cleaved caspase3, BcL-xL and c-Myc, Stat3, Cyclin D1 and VEGF gene level in DU145 cells transfected with different vectors. (c) Tumor-associated protein expression in DU145 cells transfected with psi-Survivin, pGRIM-19, pGRIM-19-si-Survivin and psi-Scramble empty vector revealed by Western blot. (d) The quantified cleaved caspase3, BcL-xL, C-Myc, Stat3 and cyclin D1 protein levels in DU145 cells transfected with different treatment vectors. Optical density was expressed as mean±s.e. (*P<0.05, **P<0.01 versus mock; §P<0.05, §§P<0.01 versus psi-Scramble group; #P<0.05, # # P<0.01 versus psi-Survivin; ‖‖P<0.05, ‖‖‖‖P<0.01 versus pGRIM-19 group). BcL-XL, B-cell lymphoma xL; GRIM-19, gene associated with retinoid-interferon-induced mortality-19; RT-PCR, reverse transcription PCR; Stat3, signal transducer and activator of transcription 3; VFGF, vascular endothelial growth factor.