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Lipofuscin deposition in rat Leydig cells was observed using flow cytometer. Leydig cells isolated from chronic stress rats displayed a prominent increase in cellular autofluorescence compared to cells from young control rats, approximately sixfold after 40 days of chronic stress. Leydig cells isolated from old rats displayed on average a >10-fold increase in autofluorescence comparison to Leydig cells from young control rats, consistent with senescence.
