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. 2012 May 21;14(4):643–648. doi: 10.1038/aja.2011.183

Figure 6.

Figure 6

DNA damage in Leydig cells was observed using the comet assay. (a) Image of Leydig cell nucleoids using the comet assay; cells were isolated from 6-month-old rats. The limited migration of DNA toward the anode indicates the cells have a low level of DNA damage. (b) Image of Leydig cell nucleoids; cells were isolated from rats exposed to chronic stress. The significant migration of DNA into the tail of the comet indicates greater DNA damage. (c) Image of Leydig cell nucleoids; cells were isolated from 21-month-old rats. DNA breaks within long loops of DNA allow them to migrate toward the anode, giving rise to a comet-like appearance in heavily damaged nucleoids. (d) DNA damage was measured using the comet assay and analysed using Comet 5.0 in Leydig cells of the three groups. *indicate a significant difference compared with the Young group (P<0.05).