Table 2. Sperm parameters before and after each freezing/thawing cycle(n=20).
| Cycle of freezing/thawing (F/T) | |||||
|---|---|---|---|---|---|
| Parameters | Pre-freeze | Cycle 1 | Cycle 2 | Cycle 3 | Pa |
| Viability (%) | 85.6±9.2 | 68.1±6.4 | 52.3±9.9 | 38.9±2.4 | <0.001 |
| Morphology (%) | 20.6±6.1 | 18.6±6.2 | 18.0±4.4 | 17.5±5.8 | 0.018 |
| Motility (%) | 81.3±8.2 | 66.3±9.4 | 37.9±15.3 | 25.1±7.2 | <0.001 |
| VAP | 69.2±13.4 | 61.9±12.7 | 48.3±13.1 | 50.4±16.1 | <0.003 |
| VSL | 56.4±9.2 | 52.0±11.8 | 40.8±13.4 | 43.1±15.9 | <0.003 |
| VCL | 120.2±34.9 | 107.6±20.6 | 87.7±17.9 | 96.7±26.5 | <0.003 |
| ALH | 5.3±1.2 | 5.0±0.8 | 4.2±1.5 | 4.5±1.9 | 0.089 |
| LIN | 49.1±7.9 | 49.0±7.4 | 46.1±9.6 | 45.9±11.9 | 0.764 |
| Comet assay | |||||
| Head DNA (%) | 92.9±5.9 | 91.0±6.5 | 84.0±8.5 | 75.5±12.8 | <0.001 |
| Tail DNA (%) | 7.2±5.9 | 9.0±6.5 | 15.9±8.5 | 24.5±12.8 | <0.001 |
| Tail moment | 13.6±16.0 | 25.4±20.8 | 32.6±18.9 | 63.0±37.7 | <0.001 |
Abbreviations: ALH, amplitude of lateral head displacement (µm); LIN, linearity (%); VAP, average path velocity (µm s−1); VCL, curvilinear velocity (µm s−1); VSL, straight line velocity (µm s−1).
Values are expressed mean±s.d.
a
Friedman nonparametric repeated measure tests.