Fig. 4.

Responses of Ca²⁺ transient measures to different Ca²⁺ channel subtype blockers in small (A) and large (B) control sensory neurons stimulated by high K⁺. Mibefradil (Mib, 200 nm), nicardipine (Nic, 10 μm), SNX-111 (“111,” 200 nm), ω-agatoxin-IVA (Aga, 200 nm), and SNX-482 (“482,” 100 nm) were applied. Each neuron was exposed to only a single agent. An asterisk within the bar indicates a significant effect on the transient after application compared with baseline. There was a significant main effect between agents for each measure in each cell size category. Brackets indicate significant differences by post hoc paired comparisons (Bonferroni). Area = transient area; R = ratio of fluorescence excited by 340 nm divided by fluorescence excited by 380 nm; R_BLΔ = offset of the posttransient baseline above the original baseline; RΔ_max = transient amplitude. Numbers in parentheses (n) apply to each parameter for that neuronal size. Note that the scales are different in the positive and negative direction for Area in large neurons.