Figure 2.

Age-dependent behavioral impairment in TgF344-AD rats. A, Schematic of plasmids coinjected into Fischer rat pronuclei to generate TgF344-AD rats, adapted from the study by Jankowsky et al. (2001). B, PCR results from APPsw, PS1ΔE9, or TcRδ reactions. C, Western blots for brain APP, PS1ΔE9, PS1 N-terminal fragment (NTF), or actin are shown. D, Densitometric analysis of Western blots from WT or transgenic rat brains are shown (n = 12) relative to actin. Fold increases across genotype are indicated. E–I, Six groups of littermate rats, including both WT and Tg animals aged 6 months (WT, n = 21; Tg, n = 21), 15 months (WT, n = 28; Tg, n = 30), or 24 months (WT, n = 21; Tg, n = 20), were subjected to behavioral testing. E, F, Total movement (by rearing or beam breaks) was evaluated in an open field in 5 min bins over 60 min. G, Preference for a novel versus a familiar object is shown. H, I, Errors made during Barnes maze testing are shown. All data are represented as means + SEM.