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AF (A, D), SHG (B, G), and eosin-labeled fluorescence (Eosin, [E, H]) in human CS. Merged images are (C) AF and SHG, (F) AF and Eosin, and (I) SHG and Eosin. (A–C) AF-low coincided with SHG signals (asterisks), but AF-high (arrowheads) coincided with SHG signal-voids (arrows). (D–F) AF-high, but not AF-low coincided with EOS-pos. (G, H) EOS-pos coincided with SHG signal-voids. Scale bars: 10 μm.
