Table 2.
Synthetic peptides used to develop antibody against YABBY and NAC transcription factor
|
TF name |
Gene model |
Amino acid position |
Sequence of synthetic peptides |
|---|---|---|---|
| Used for antibody production | |||
| NAC |
Glyma12g04380.1* |
13-26 |
VRTGGKGTMRRKKK |
| Glyma11g12170.1 | |||
| Glyma11g12180.1* Glyma01g18780.1 | |||
| YABBY |
Glyma13g22620.1 |
99-112 |
TEERVVNRPPEKRQ |
| Glyma17g12200.1 |
All the antibodies are produced by GenScript Corporation. They used James and Wolf (JW) prediction algorithm to design synthetic peptides for the production of antibody against YABBY and NAC transcription factor.
* Used for single amino acid difference between Glyma12g04380.1 and synthetic peptide at amino acid position 21 where Valine was replaced with Methionine (M).