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. 2013 Jul 23;8(7):e69387. doi: 10.1371/journal.pone.0069387

Figure 4. PRRSV-infection and nsp4-transfection activated caspases.

Figure 4

(A, B, and C) PAMs were mock infected or infected with HP–PRRSV (HV strain) at an MOI of 0.5. At 24 hours post infection, the enzymatic activities of caspase-3, -8, and -9 were examined using colorimetric assays. (D) Hela cells were transfected with pCMV-Myc control or nsp4-expressing plasmid. At 48 hours post transfection, activated caspase 3 (17 kDa), cleaved PARP, and nsp4 were analyzed using western blot. (E, F and G) Hela cells were transfected with pCMV-Myc control or nsp4-expressing plasmid. At 48 hours post transfection, the enzymatic activities of caspase-3, -8, and -9 were examined using colorimetric assays. Data represent means ± SD of three independent experiments. P<0.05 (*), P<0.01 (**), P<0.001 (***) as determined by student’s t test.