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| Name | Sequence |
|---|---|
| N69 forward | 5'-GCGTCGACAATGTGCCCGAATTGGCAAG-3' |
| N69 reverse2 | 5'-ATTTGCGGCCGCTCATTCCAGTTCAGGTTTGGCA-3' |
| MID forward-3 | 5'-TGGGCTTCACAGTCCACC-3' |
| MID reverse-3 | 5'-GTGAAGCCCATCAAGCTGAG-3' |
| C157 forward1 | 5'-GCGTCGACCATGGGTGCTTTCAGAACTCAA-3' |
| C157 reverse | 5'-ATTTGCGGCCGCTCAGGGCTTCACATTACAAAAC-3' |
1 The forward primer of MID and C157 deletion mutants.
2 The reverse primer of N69 and MID deletion mutants.
