Table 2. Sequences of the primers used for NSP4 protein piont mutants.
| Name | Sequence |
|---|---|
| C157 forward1 | 5'-GCGTCGACCATGGGTGCTTTCAGAACTCAA-3' |
| H-A reverse-3 | 5'-CCGTAAGGACTGCTGCGGC-3' |
| H-A forward-3 | 5'-GTCCTTACGGGTAACTCAGCTAG-3' |
| D-A reverse-3 | 5'-CTATGGCGAATGCCCCTTTTAC-3' |
| D-A forward-3 | 5'-TTCGCCATAGCTGATTGCCC-3' |
| S-A reverse-3 | 5'-CTGGGGATCCTGCATCGCCACAC-3' |
| S-A forward-3 | 5'-GGATCCCCAGTGATTACCGAAG-3' |
| N69 reverse2 | 5'-ATTTGCGGCCGCTCATTCCAGTTCAGGTTTGGCA-3' |
1 The forward primer of H39A, D64A and S118A piont mutants.
2 The reverse primer of H39A, D64A and S118A piont mutants.