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. 2013 Jul 4;13:157. doi: 10.1186/1472-6882-13-157

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Copyright © 2013 Ling-Sing Seow et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of the specific inhibitors of MEK/ERK1/2 and PI3K on aqueous extracts-stimulated neuritogenesis. PC-12 cells were exposed to U0126, PD98059 and LY294002 for 1 h before the treatment of aqueous extracts of G. lucidum (GL), G. neo-japonicum (GNJ) and G. frondosa (GF). Cells in complete F-12 K medium without treatment served as a negative control. Cells treated with 50 ng/ml of NGF or 50 μg/ml of H. erinaceus (HE) served as positive controls. A control without inhibitor was used in each treatment group. Data were expressed as means ± standard deviation (n = 3). *** p < 0.001 compared to respective controls.