Fig. 2.

Expression of C and V proteins from SeV P gene and recovery of knockout viruses; V(-) with the entire V protein deleted, VΔC with the V-unique region deleted and 4C(-) with all four C proteins (C′, C, Y1 and Y2) deleted. For details see the text. Deletion of the respective proteins was verified by Western blotting of cells infected with the recovered knockout viruses. Wt, cells infected with wild-type SeV. The dotted line in the insert indicates deletion. (Adapted from Ref. 7 with permission.)