Figure 6. HDM induced upregulation of VEGF-A and CXCL1 in macrophages is HIF-1α dependent.

A. Representative photomicrographs of lungs from mice treated with PBS (top panel) or HDM (bottom panel) for 1 week, immunofluorescently stained for HIF-1α (green) and counter stained with DAPI (blue). Original magnification 400×. Isolated macrophages from lung tissue and were stimulated with HDM in vitro, for 24 h, in the presence or absence of HIF-1α blockade. CXCL1 (A) and VEGF-A levels were measured by ELISA in the culture supernatants. Expression of PHD1 and PHD3 (D) was assessed by western blotting of cell lysates. Bars represent mean ± SEM. Data derived from 3 independent experiments. *represents p<0.05, **represents p<0.01.