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. 2013 Jul 23;8(7):e69644. doi: 10.1371/journal.pone.0069644

Figure 2. Assay of rVpmaX adhesion and adhesion inhibition to EBL cells visualized by confocal laser scanning microscopy.

Figure 2

Active rVpmaX interacted with fixed EBL cells, and the surplus protein was rinsed away by washing with PBST. The attached protein was immunostained with rabbit anti-rVpmaX antibody and mouse anti-rabbit IgG-FITC. The EBL cell membranes were labeled with 1,19-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI), and the cell nuclei were counter-labeled with 49,6-diamidino-2-phenylindole (DAPI). (A1–A2) 10 µg rVpmaX adhering to EBL cells. (B) Adhesion inhibition of 10 µg rVpmaX to EBL cells by 10 µl rabbit anti-rVpmaX serum. (C) Adhesion of 20 µg rVpmaX to EBL cells. (D) The adhesion of 20 µg rVpmaX to EBL cells was inhibited by 20 µl rabbit anti-rVpmaX serum. (E) EBL cells without protein added.