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. 2013 Jul 23;8(7):e69286. doi: 10.1371/journal.pone.0069286

Figure 3. Thy1-p45 transgenic mice display decreased cell death following stab wound injury of the spinal cord.

Figure 3

(A–D) Brain (A, C) and spinal cord (B, D) sections from WT littermates (A, B) and Thy1-p45 transgenic mice (C, D) were immunostained with an anti-p45 antibody. Ctx (V, VI), cortex layers V&VI; BLA, basolateral amygdala; DG, dentate gyrus; Thal, thalamus; fx, fibers of fornix; DH, dorsal horn; VH, ventral horn; grf, gracile fasiculus; dcs, dorsal corticospinal; *, spinal projection fibers from dorsal root ganglia neurons. Scale bar, 1 mm (A, C); 200 µm (B, D). (E–F) WT and Thy1-p45 mice were subjected to stab wound injury in the right side of the spinal cord at the T13 level. One and 3 days after injury, sections were stained for TUNEL+ (E) and activated caspase-3+ (F) cells. The numbers of TUNEL+ and activated caspase-3+ cells were counted in the half spinal cord contralateral (C) and ipsilateral (I) to the stab wound injury. The numbers of TUNEL+ and activated caspase-3+ cells in the ipsilateral side were markedly lower in the Thy1-p45 mice. (G) Six weeks after stab wound injury, the number of NeuN+ cells within 1 mm from injury site was significantly higher in the Thy1-p45 mice compared to their WT littermates, suggesting that p45 expression may decrease neuronal cell death. *P<0.01. ***P<0.0001. ns, not significant. Data are represented as means ± s.e.m.